Bordetella pertussis/parapertussis DNA, Qualitative, Real-Time PCR

M101

Methodology:Real-Time Polymerase Chain Reaction (PCR)
Performed:Mon-Fri
Reported:3-7 days
Specimen Required:Collect: Preferred Specimen(s): Nasopharyngeal aspirate or nasopharyngeal swab 
Alternative Specimen(s): Nasopharyngeal swab submitted in a VCM medium (green-cap) tube or equivalent UTM
• Multi Microbe Media (M4)
• eSwabs 
Specimen Preparation:  
Nasopharyngeal aspirates: Instill 1-1.5 mL of nonbacteriostatic saline (pH 7.0) into one nostril. Flush a plastic catheter or tubing with 2-3 mL of saline. Insert the tubing into the nostril parallel to the palate. Aspirate nasopharyngeal secretions. Repeat this procedure for the other nostril. Combine aspirates into a sterile vial.
Nasopharyngeal swab: Collection kits using Amies liquid, M4, VCM/UTM are acceptable. Swab tips may be polyester, rayon, nylon flocked. Flexible shafts must be used for proper collection. Accuracy of the test depends on the collection of ciliated epithelial cells material. Half the distance from the nostril to the base of the ear could be used as estimation of the site of collection. Gently insert swab into the nostril. Keep the swab near the septum floor of the nose while gently pushing into the posterior nasopharynx. Rotate swab 2-3 times and hold in place for a few seconds for optimal collection. Collection of both nostrils is recommended but not required.
Storage/Transport Temperature: Refrigerated 
Reject Criteria: Collection tubes with Amies gel or charcoal containing media • Samples collected using cotton or calcium alginate tipped swabs • Rigid shaft swabs 
Stability:Nasopharyngeal aspirate
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: 30 days 
Nasopharyngeal swab
Room temperature: 7 days
Refrigerated: 7 days
Frozen: 30 days
NOTE: * This test is approved for all states. * 
Performing Laboratory 
Quest Diagnostics Nichols INST 
CPT Code(s):87798×2

Find a Location

Cranford Patient Service Center

  • 27 South Avenue West, Cranford, NJ 07016
  • (908) 441-7710
  • (908) 441-7711
  • Mon., Tue., Wed.: 7:00 AM – 6:00 PM
  • Thu., Fri.: 7:00 AM – 5:00 PM
  • Sat.: 8:00 AM – 12:00 PM
  • Sun.: CLOSED

Linden Patient Service Center

  • 1901 East Linden Avenue, Unit 25, Linden, NJ 07036
  • (908) 474-1004
  • Mon. – Fri.: 8:00 AM – 2:00 PM
  • Sat., Sun.: CLOSED

Somerset Patient Service Center

  • 1553 Rt.27, Somerset, NJ 08873
  • (609) 527-9199
  • (609) 527-9192
  • Mon. – Fri.: 9:00 AM – 5:00 PM
  • Sat., Sun.: CLOSED

Succasunna Patient Service Center

  • 225 State Route 10, Succasunna, NJ 07876
  • (973) 971-9314
  • Mon. – Fri.: 9:00 AM – 5:00 PM
  • Sat., Sun.: CLOSED

Voorhees Sheppard Patient Service Center

  • 903 Sheppard RD., Voorhees, NJ 08043
  • (856) 528-5915
  • Mon. – Fri.: 8:00 AM – 5:00 PM
  • Sat., Sun.: CLOSED

Stamford Patient Service Center

  • 111 High Ridge Road, First Floor, Stamford, CT 06905
  • (908) 583-6632
  • Mon., Wed., Fri.: 9:00 AM – 3:00 PM
  • Tue., Thu., Sat., Sun.: CLOSED
  • Lunch Break: 12:00 PM – 12:30 PM

East Hartford PSC

  • 677 Silver Ln, East Hartford, CT 06118
  • (860) 216-2150
  • (860) 904-9638
  • Mon., Fri.: 9:00 AM – 3:00 PM
  • Sat., Sun.: CLOSED

Baltimore Patient Service Center

  • 3455 Wilkins Avenue, Suite L10-A, Baltimore, MD 21229
  • (410) 644-4444
  • Mon., – Fri.: 8:00 AM – 4:00 PM
  • Sat., Sun.: CLOSED

Cambridge Patient Service Center

  • 503 Byrn Street, 2nd Floor, Cambridge, MD 21613
  • (443) 477-6568
  • (443) 477-6530
  • Mon., – Fri.: 6:00 AM – 1:00 PM
  • Sat., Sun.: CLOSED

Greenbelt Patient Service center

  • 7525 Greenway Center Drive, Suite T9, Greenbelt, MD 20770
  • (856) 454-5128
  • (856) 454-5136
  • Mon., – Tue.: 7:00 AM – 5:00 PM
  • Wednesday: 7:00 AM – 7:00 PM
  • Thursday: 7:00 AM – 5:00 PM
  • Friday: 7:00 AM – 6:00 PM
  • Saturday: 8:00 AM – 4:00 PM
  • Sun.: CLOSED

5th Avenue Patient Service Center

  • 5908 5th Avenue, Brooklyn, NY 11220
  • (718) 880-9970
  • Mon., – Thu.: 10:00 AM-6:00 PM
  • Fri.: 10:00 AM-1:00 PM
  • Sat.: 10:00 AM-6:00 PM
  • Sun.: CLOSED

First Medcare

  • 8707 Flatlands Avenue, Lower Level, Brooklyn, NY 11236
  • (718) 210-1909
  • (718) 210-1910
  • (718) 210-1910
  • Mon., Tue., Thu., Fri.: 8:00 AM – 4:30 PM
  • Wed.: 10:00 AM – 6:00 PM
  • Sat., Sun.: CLOSED

New Hyde Park Patient Service Center

  • 1575 Hillside Avenue, New Hyde Park, Suite 100-A, NY 11040
  • (516) 500-1047
  • Mon., – Fri.: 7:30 AM-4:00 PM
  • Sat., Sun.: CLOSED

Rockville Centre Patient Service Center

  • 178 Sunrise Hwy, Lower Level, Rockville Centre, NY 11570
  • (516) 442-0417
  • (516) 442-0418
  • Mon., – Fri.: 7:00 AM-7:00 PM
  • Sat.: 8:00 AM-2:00 PM
  • Sun.: CLOSED

Haverstraw Patient Service Center

  • 52 New Main Street, Haverstraw, NY 10927
  • (845) 310-4659
  • (845) 310-4660
  • Mon., – Thu.: 7:30 AM-4:30 PM
  • Fri., Sat.: 7:00 AM-1:00 PM
  • Sun.: CLOSED

Medical Drive Patient Service Center

  • 1314 Medical drive, Suite 101, Fayetteville, NC 28304
  • (910) 491-5253
  • Mon., – Thu.: 8:00 AM-5:00 PM
  • Fri.: 8:00 AM-2:00 PM
  • Sat., Sun.: CLOSED

Skibo Road Patient Service Center

  • 1905 Skibo Road, Suite 101, Fayetteville, NC 28314
  • (910) 867-7071
  • Mon., – Fri.: 8:00 AM-6:00 PM
  • Sat., Sun.: CLOSED

Walter Reed Patient Service Center

  • 1220 Walter Reed Road, Suite A, Fayetteville, NC 28304
  • (910) 481-1956
  • Mon., – Thu.: 7:00 AM-4:00 PM
  • Fri.: 8:00 AM-2:00 PM
  • Sat., Sun.: CLOSED

Bangor Patient Service Center

  • 225 Erdman Avenue, Bangor, PA 18013
  • (610) 452-9174
  • Mon., – Fri.: 8:00 AM – 1:00 PM
  • Sat., Sun.: CLOSED

Hazelton Patient Service Center

  • 101 South Church Street, Hazelton, PA 18704
  • (570) 455-0747
  • Mon., – Fri.: 8:00 AM – 4:00 PM
  • Sat., Sun.: CLOSED

Kingston Patient Service Center

  • 517 Pierce Street, Kingston, PA 18704
  • (570) 283-0674
  • Sat., Sun.: CLOSED

ACCU Reference Louisiana Stat LAB

  • 1532 River Oaks Road W., New Orleans, LA 70123
  • (504) 779-8163
  • (504) 779-8164
  • Mon., – Fri.: 7:00 AM – 4:00 PM
  • Sat., Sun.: CLOSED

Metairie Patient Service Center

  • 4213 Saxon Street, Suite #100, Metairie, LA 70006
  • (504) 516-2461
  • Mon., – Fri.: 7:00 AM – 4:00 PM
  • Sat., Sun.: CLOSED

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