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Acid-Fast Bacillus (AFB) Culture and AFB Stain
A982
| Methodology: | Stain/Culture/Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) Mass Spectrometry/16SrDNA Sequencing/Polymerase Chain Reaction/Broth Microdilution |
| Performed: | Sun-Sat |
| Reported: | 1-62 days from receipt of specimen Stain reported within 24 hours. Negative cultures reported at 8 weeks. Positive cultures are reported as soon as detected. |
| Specimen Required: | Patient Preparation: Recommended collection: Three sputum specimens at 8-24 hour intervals (24 hours when possible) and at least one first-morning specimen. An individual order must be submitted for each specimen. Collect: Respiratory specimens. Also acceptable: Body fluid, CSF, tissue, or urine. Specimen Preparation: Respiratory Specimens: Transfer (for each collection) 5-10 mL to a sterile container. (Min: 1 mL) Body Fluids: Transfer 5 mL to a sterile container. (Min: 1 mL culture only) CSF: Transfer 5 mL to a sterile container. (Min: 1 mL culture only. Min: 5 mL culture and stain) Tissue: Transfer to a sterile container. (Min: Visible, for small tissue that cannot be ground, acid fast stain will not be performed.) Urine: Transfer at least 40 mL to a sterile container. (Min: 10 mL culture only. Min: 40 mL culture and stain) Remarks: Specimen source required. Storage/Transport Temperature: Refrigerated. Unacceptable Conditions: Dry material or material collected and transported on a swab. Acid Fast Stain: Stool, blood, bone marrow, grossly bloody specimens. Stability: Ambient: 24 hours; Refrigerated: 1 week; Frozen: 2 weeks Note: * This test is approved for all states. * Performing Lab ARUP Laboratories |
| CPT Code(s): | 87116; CPT codes for identification and susceptibility vary based on method. |